lntroductory Cell Biology & Genetics- LAB

REQUIRED TEXTBOOKS AND COURSE MATERIALS

Required Textbook: Reecy and Urry. Biology Lab Manula. Pearson Collections

DESCRIPTION

An introductory laboratory course focusing on scientific inquiry and investigation of cell and molecular biology principles, use of laboratory equipment, and metric system measurements and conversions.
Pre- and post-lab activities: Labs are an opportunity to gain hands-on experience with the theories, organisms and processes that we discuss in lecture. To prepare you for each lab, you will complete a short pre-lab quiz that will evaluate class reading of the lab's goals and tasks.
Post-lab assignments will assess your understanding of key observations and concepts covered in the lab activity and connect them to lecture content. Pre-lab activities will be completed individually on Collab, while post-lab activities will usually be completed as a group and handed in at the end of each lab activity.

OUTLINE

Session 1: Introduction to Biology Science
Session 2: and 3- The cell: different cell types and how to observe them
Session 4: The cell II. Microscopy
Session 5: Macromolecules. Metabolism. Fermentation
Session 6: Photosynthesis and Climate Change
Session 7: Photosynthesis: Separation of Photosynthetic Pigments Using Paper Chromatography
Session 8: Mitosis: Investigating root tip mitosis
Session 9: Meiosis in anthers of eggplant
Session 10: GENETICS: How to preserve genetic variability
Session 11: DNA extraction (Do it yourself –DIY-)
Session 12: Karyotyping

STUDENT LEARNING/COURSE OUTCOMES

-Stimulating curiosity and critical thinking and at the same time surprising and amusing the public by its contents from the world of science, technology, and the environment.
-Learn about the light microscope and how to use it to enlarge the image of cells. Observe different cell types and review the main parts of a cell.
- Microscopes and lenses. Use of light microscope
- Prepare temporary slides for microscopic observation
- Prokaryotic cells: environmental bacterial cultures
- Unicellular eukaryotic cells: organisms in stagnant water
- Vegetal eukaryotic cells: onion epithelium
- Animal eukaryotic cells fly wings cells
- Human eukaryotic cells: buccal epithelium
- Use a light microscope to observe slides of prokaryotic cells, fungi, animals and unicellular algae
- Differentiate eukaryotic and prokaryotic cells
- Recognize basic structures of the cell
-Identify cell types and components of cell walls and membranes with the scanning electron microscope.
-To use a technique called paper chromatography to separate chemical compounds of a mixture.
- Prepare temporary slides of cells found in garlic root tip meristem tissue
- Use a light microscope to observe, identify and draw root tip meristem cells in different stages of the cell cycle
- Measure the sizes of some of these cells
- Calculate the mitotic index for the garlic root tip tissue that you observe
- Use a light microscope to observe mitosis slides from different kingdoms. Chromosome observation
- Prepare temporary slides of follicular testes of grasshopper males/ anthers of eggplant
- Use a light microscope to observe, identify and draw identify the main stages of meiosis.
- Use a light microscope to observe meiosis slides from different kingdoms.
- Identify the differences between mitosis and meiosis
-isolate their own DNA and DNA from other organisms
-To make a karyotyping using several templates to identify chromosome disorders.