Molecular Cell Biology

COURSE OBJECTIVE
To obtain a basic understanding of the functioning of living cells at the molecular level, and introduction to qualitative and quantitative methods applicable inside and outside the living cell. To learn to design and execute studies where these methods are applied, and to interpretate the results.

Specifically, the students will be trained in:

• The use and interpretation of information about cellular processes such as energy metabolism, regulation of gene expression, oxygen damage, apoptosis and signal transduction.
• The design and execution of simple experiments concerning gene expression, signal transduction, growth and metabolic processes.
• Application of Metabolic Control Analysis to quantify distribution of control and regulation of cellular processes by individual cellular reactions.
• The use of existing computer models of molecular systems in the cell to explore the behaviour of these systems.

COURSE CONTENT

• Introduction to Metabolic Control Analysis and its basis in enzyme kinetics.
• Laws and practical applications of Metabolic Control Analysis, and extension of this method to include gene expression.
• Modular kinetic analysis of cellular networks.
• Structure and function of respiratory networks, with Paracoccus denitrificans as example.
• Regulation of gene expression during nitrification and denitrification in microorganisms.
• Use of a depository of mathematical models to explore reaction systems in the cell.
• Control Analysis of signal transduction as a key in the understanding of cancer..
• Application of the presented methods to a case study: fighting the parasite that causes sleeping sickness.

TEACHING METHODS
Lectures; workgroups; computer practical; practical project in the department of Molecular Cell Physiology

TYPE OF ASSESSMENT
Written exam (70%) Presentation (30%)

ENTRY REQUIREMENTS
Participation in the course “From Protein to Cell” (1st half of period 2)

TARGET AUDIENCE
Part of the minor Biomolecular Science and Neuroscience, track Biomolecular Science.

REMARKS
For the practical a laboratory coat will be supplied. After the course, the coat will be sterilised. Participation in the course "From Protein to Cell" (1st half of period 2) is compulsory.